pax9  (OriGene)

Journal: Journal of Dental Research

Article Title: Nine Novel PAX9 Mutations and a Distinct Tooth Agenesis Genotype-Phenotype

doi: 10.1177/0022034517729322

Figure Lengend Snippet: Summary of PAX9 Mutations in Patients with Tooth Agenesis.

Article Snippet: The human PAX9 complementary DNA (cDNA) (accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_006194","term_id":"260656050","term_text":"NM_006194"}} NM_006194 ; Origene) was subcloned into the pCMV-C-Myc expression vector (Beyotime) between 5′-BamHI and 3′-EcoRI sites to generate the pCMV-PAX9-Myc wild-type plasmid.

Techniques: Mutagenesis

Journal: Journal of Dental Research

Article Title: Nine Novel PAX9 Mutations and a Distinct Tooth Agenesis Genotype-Phenotype

doi: 10.1177/0022034517729322

Figure Lengend Snippet: Clinical characteristics of probands with a PAX9 mutation. (A) Intraoral photos and panoramic radiographs of probands with distinct PAX9 mutations. Asterisks represent the position of missing teeth. (B) The prevalence of missing teeth in all PAX9 mutation patients (n = 16). L, lower; U, upper. (C) The prevalence of microdontia in all patients with a PAX9 mutation.

Article Snippet: The human PAX9 complementary DNA (cDNA) (accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_006194","term_id":"260656050","term_text":"NM_006194"}} NM_006194 ; Origene) was subcloned into the pCMV-C-Myc expression vector (Beyotime) between 5′-BamHI and 3′-EcoRI sites to generate the pCMV-PAX9-Myc wild-type plasmid.

Techniques: Mutagenesis

Journal: Journal of Dental Research

Article Title: Nine Novel PAX9 Mutations and a Distinct Tooth Agenesis Genotype-Phenotype

doi: 10.1177/0022034517729322

Figure Lengend Snippet: Location and conservation analysis of tooth agenesis associated PAX9 mutations. (A) Distribution of mutations identified in patients with tooth agenesis in the PAX9 protein. Reported mutations are labeled in blue. Black indicates novel PAX9 mutations. (B) Schematic diagram of the PAX9 gene. (C) Conservation analysis of affected amino acids in the PAX9 protein among 9 different vertebrate species.

Article Snippet: The human PAX9 complementary DNA (cDNA) (accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_006194","term_id":"260656050","term_text":"NM_006194"}} NM_006194 ; Origene) was subcloned into the pCMV-C-Myc expression vector (Beyotime) between 5′-BamHI and 3′-EcoRI sites to generate the pCMV-PAX9-Myc wild-type plasmid.

Techniques: Labeling

Journal: Journal of Dental Research

Article Title: Nine Novel PAX9 Mutations and a Distinct Tooth Agenesis Genotype-Phenotype

doi: 10.1177/0022034517729322

Figure Lengend Snippet: Functional studies of mutant PAX9 proteins. (A) Expression of wild-type and mutant PAX9 proteins detected by Western blot. (B) Relative messenger RNA (mRNA) expression levels of mutant PAX9 at 12 h posttransfection. (C) After treatment with actinomycin D, PAX9 mRNAs underwent decay. The mRNA stability is presented by the percentage of remaining mRNAs at 4 and 8 h posttreatment. *P < 0.05. (D) Subcellular localization of wild-type and mutant PAX9 proteins assessed by immunofluorescence. (E) The transcriptional activation abilities of PAX9 mutants on the BMP4 promoter assessed by luciferase reporter assay. **P < 0.01. (F) Binding of PAX9 mutants to the paired domain consensus sequence CD19-2 (A-ins) assessed by an electrophoretic mobility shift assay.

Article Snippet: The human PAX9 complementary DNA (cDNA) (accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_006194","term_id":"260656050","term_text":"NM_006194"}} NM_006194 ; Origene) was subcloned into the pCMV-C-Myc expression vector (Beyotime) between 5′-BamHI and 3′-EcoRI sites to generate the pCMV-PAX9-Myc wild-type plasmid.

Techniques: Functional Assay, Mutagenesis, Expressing, Western Blot, Immunofluorescence, Activation Assay, Luciferase, Reporter Assay, Binding Assay, Sequencing, Electrophoretic Mobility Shift Assay